Charles Ellsworth Tuffley (December 5, 1872 – September 3, 1956) was an American farmer and politician.

Tuffley was born on a farm in Grant County, Wisconsin. He lived in Boscobel, Wisconsin and raised Holstein cattle. Tuffley served in the Wisconsin Assembly from 1921 to 1926 and was a Republican.Wisconsin Blue Book, 1925, Biographical Sketch of Charles E. Tuffley, pp. 663–664. He served on committees for elections and highways. Tuffley died at a hospital in Boscobel, Wisconsin.Obituaries-Dubuqueland Deaths-Charles A. Tuffley, Dubuque Telegraph-Herald, September 4, 1956, p. 12.

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