List of restriction enzyme cutting sites: Bsp–Bss

This article contains a list of the most studied restriction enzymes whose names start with Bsp to Bss inclusive. It contains approximately 180 enzymes.

The following information is given:

__TOC__

Whole list navigation

Restriction enzymes

Bsp

Enzyme PDB code Source Recognition sequence Cut IsoschizomersBsp6I Bacillus sp. RFL6 5' GCNGC 3' CGNCG 5' ---GC NGC--- 3' 3' ---CGN CG--- 5' Bsp13I Bacillus sp. 13 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' AccIII, BbvAIII, BlfI, BsiMI, BspMII, Bsu23I, Kpn2I, PinBIIBsp19I Bacillus sp. 19 5' CCATGG 3' GGTACC 5' ---C CATGG--- 3' 3' ---GGTAC C--- 5' Bsp24I Bacillus sp. 24 5' GACN6TGG 3' CTGN6ACC 5' ---GACN6TGGN6NNNNNN --- 3' 3' ---CTGN6ACCN6N NNNNN--- 5' Bsp50I Bacillus sp. RFL50 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' BceBI, Bsh1236I, BstFNI, BstUI, Bsu1532I, FalII, FnuDII, MvnI, ThaIBsp63I Bacillus sphaericus 63 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AjoI, Asp713I, BloHII, MhaAI, Pfl21I, PstI, SalPI, SflI, Sst12I, YenIBsp67I Bacillus sphaericus 67 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' AspMDI, Bsp143I, BspJI, BtkII, DpnII, LlaAI, NlaII, SsiBIBsp68I Bacillus sp. RFL68 5' TCGCGA 3' AGCGCT 5' ---TCG CGA--- 3' 3' ---AGC GCT--- 5' NruIBsp98I Bacillus sp. RFL98 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' AccEBI, AliI, BamHI, BnaI, BstI, CelI, OkrAI, NspSAIV, Pfl8I, SurIBsp105I Bacillus sphaericus 105 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bfi57I, Bsp143I, BspJI, BtkII, DpnII, LlaAI, NlaII, SsiBIBsp106I Bacillus sphaericus 106 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BbvAII, Bci29I, BciBI, BcmI, BspOVII, BstNZ169I, Rme21IBsp119I Bacillus sp. RFL119 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' AcpI, Bim19I, Bpu14I, BspT104I, Csp45I, FspII, NspV, SspRFIBsp120I Bacillus sp. RFL120 5' GGGCCC 3' CCCGGG 5' ---G GGCCC--- 3' 3' ---CCCGG G--- 5' ApaI, PpeI, PspOMIBsp123I Bacillus sp. 123 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' BceBI, Bsh1236I, BstFNI, BstUI, BtkI, Csp68KVI, FnuDII, MvnI, ThaIBsp143I Bacillus sp. RFL143 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bce243I, Bsp105I, BspFI, BstKTI, CpfI, HacI, NdeII, Sth368I, Sau3AIBsp143II Bacillus sp. RFL143 5' RGCGCY 3' YCGCGR 5' ---RGCGC Y--- 3' 3' ---Y CGCGR--- 5' AccB2I, BfoI, Bme142I, BstH2I, HaeII, LpnIBsp211I Bacillus sphaericus 211 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' Bsp423I Bacillus sp. 423 5' GCAGC 3' CGTCG 5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5' AlwXI, BbvI, BseKI, BseXI, Bst12I, Bst71I, BstV1IBsp519I Bacillus sp. 519 5' GRGCYC 3' CYCGRG 5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5' Bsp1286I Bacillus sphaericus 5' GDGCHC 3' CHCGDG 5' ---GDGCH C--- 3' 3' ---C HCGDG--- 5' AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, NspII, SduIBsp1407I Bacillus stearothermophilus RFL1407 5' TGTACA 3' ACATGT 5' ---T GTACA--- 3' 3' ---ACATG T--- 5' AauI, BsmRI, BsrGI, BstAUI, Ssp4800I, SspBIBsp1720I Bacillus sp. 1720 5' GCTNAGC 3' CGANTCG 5' ---GC TNAGC--- 3' 3' ---CGANT CG--- 5' Bsp1894I Bacillus sphaericus 1894 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AspS9I, Bac36I, BavBII, BshKI, BspBII, CcuI, MaeK81II, Pde12IBsp2095I Bacillus sphaericus 2095 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bfi57I, Bsp143I, BspKT6I, BtkII, DpnII, LlaAI, NlaII, SsiBIBsp4009I Bacillus sp. 4009 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' AccEBI, AliI, ApaCI, BamHI, BstI, CelI, OkrAI, Pfl8I, RspLKII, SurIBspAI Bacillus sphaericus JL4B 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bfi57I, Bsp2095I, BspKT6I, BtkII, DpnII, LlaAI, NlaII, SsiBIBspA2I Bacillus sp. A2 5' CCTAGG 3' GGATCC 5' ---C CTAGG--- 3' 3' ---GGATC C--- 5' AspA2I, AvrII, AvrBII, BlnI, XmaJIBsp153AI Bacillus sp. 153A 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' BspAAI Bacillus sp. AA 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' AbrI, BluI, BstVI, PanI, SauLPII, SciI, SlaI, StrI, TliIBspAAII Bacillus sp. AA 5' TCTAGA 3' AGATCT 5' ---T CTAGA--- 3' 3' ---AGATC T--- 5' BspAAIII Bacillus sp. AA 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' AccEBI, AliI, ApaCI, AsiI, BamHI, Bce751I, Pfl8I, RspLKII, SolIBspANI Bacillus sp. AN 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' BspBI Bacillus sphaericus JL14 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AjoI, ApiI, Asp713I, BloHII, CfrA4I, Pfl21I, PstI, SalPI, Sst12I, YenIBspBII Bacillus sphaericus JL14 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AsuI, Bac36I, BavBII, BshKI, BspF4I, CcuI, MaeK81II, PspPIBspBRI Bacillus sp. 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' BspBS31I Bacillus sp. BS31 5' GAAGAC 3' CTTCTG 5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' BspCI Bacillus sp. C1 5' CGATCG 3' GCTAGC 5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5' Afa16RI, Afa22MI, EagBI, ErhB9I, NblI, Psu161I, RshI, XorIIBspCNI Bacillus sp. 1310 5' CTCAG 3' GAGTC 5' ---CTCAGN6NNN --- 3' 3' ---GAGTCN6N NN--- 5' BspDI Bacillus sp. 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' AagI, BanIII, BavCI, Bsa29I, BseCI, Bsu15I, BsuTUI, ClaI, ZhoIBspD6I 2P14 Bacillus sp. D6 5' GACTC 3' CTGAG 5' ---GACTCNNNN NN--- 3' 3' ---CTGAGNNNNNN --- 5' BspEI Bacillus sp. 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' AccIII, BlfI, BsiMI, Bsp13I, BspMII, CauB3I, MroI, PinBIIBspFI Bacillus sp. 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bfi57I, Bsp2095I, BspKT6I, BtkII, DpnII, LlaAI, NlaII, SsiBIBspF4I Bacillus sp. F4 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AsuI, Bac36I, BavBII, BsiZI, BspBII, CcuI, MaeK81II, PspPIBspHI Bacillus sp. H3 5' TCATGA 3' AGTACT 5' ---T CATGA--- 3' 3' ---AGTAC T--- 5' BspIS4I Bacillus sp. IS4 5' GAAGAC 3' CTTCTG 5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' BspJI Bacillus sp. 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' BfuCI, Bsp2095I, BspKT6I, BtkII, DpnII, LlaAI, NmeCI, SsiAIBspJII Bacillus sp. 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' Bsa29I, BseCI, Bsp106I, BspDI, BspOVII, BspXI, BspZEIBspKI Bacillus sp. K 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' BspKT5I Bacillus sp. 5' CTGAAG 3' GACTTC 5' ---CTGAAGN12NNNN --- 3' 3' ---GACTTCN12NN NN--- 5' BspKT6I Bacillus sp. KT6 5' GATC 3' CTAG 5' ---GAT C--- 3' 3' ---C TAG--- 5' BfuCI, Bsp2095I, Bst19II, BstKTI, DpnII, LlaAI, NmeCI, SsiAIBspKT8I Bacillus sp. KT8 5' AAGCTT 3' TTCGAA 5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5' BspLI Bacillus sp. RJ3-212 5' GGNNCC 3' CCNNGG 5' ---GGN NCC--- 3' 3' ---CCN NGG--- 5' AspNI, BscBI, BmiI, NlaIV, PspN4IBspLAI Bacillus sp. LA 5' GCGC 3' CGCG 5' ---GCG C--- 3' 3' ---C GCG--- 5' AspLEI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNIBspLAII Bacillus sp. LA 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' BspLAIII Bacillus sp. LA 5' AAGCTT 3' TTCGAA 5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5' BspLS2I Bacillus sp. LS2 5' GDGCHC 3' CHCGDG 5' ---GDGCH C--- 3' 3' ---C HCGDG--- 5' AocII, BmyI, BsoCI, BspLS2I, MhlI, NspII, SduIBspLU4I Bacillus sp. LU4 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' Ama87I, AvaI, Bse15I, BsoBI, BstSI, NspIII, NspSAI, OfoIBspLU11I Bacillus sp. LU11 5' ACATGT 3' TGTACA 5' ---A CATGT--- 3' 3' ---TGTAC A--- 5' BspLU11III Bacillus sp. LU11 5' GGGAC 3' CCCTG 5' ---GGGACN8NN NNNN--- 3' 3' ---CCCTGN8NNNNNN --- 5' BspMI Bacillus sp. M 5' ACCTGC 3' TGGACG 5' ---ACCTGCNNNN NNNN--- 3' 3' ---TGGACGNNNNNNNN --- 5' Acc36I, BfuAI, BveIBspMII Bacillus sp. M 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' AccIII, BbvAIII, BlfI, BsiMI, BspEI, Bsu23I, CauB3I, MroIBspM39I Bacillus sp. M39 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' BspM90I Bacillus sp. M90 5' GTATAC 3' CATATG 5' ---GTA TAC--- 3' 3' ---CAT ATG--- 5' BspMAI Bacillus stearothermophilus MA 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AjoI, Asp713I, BloHII, PaePI, Psp23I, PstI, SalPI, Sst12I, YenIBspMKI Bacillus sp. MKI 5' GTCGAC 3' CAGCTG 5' ---G TCGAC--- 3' 3' ---CAGCT G--- 5' BspNI Bacillus sp. N 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AglI, BseBI, Bse17I, Bst2I, CbrI, CthII, EcoRII, MvaI, SspAI, TaqXIBspO4I Bacillus sp. O-4 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' BspOVI Bacillus sp. OV 5' GACN5GTC 3' CTGN5CAG 5' ---GACNNN NNGTC--- 3' 3' ---CTGNN NNNCAG--- 5' AhdI, AspEI, BmeRI, DriI, Eam1105I, EclHKI, NruGIBspOVII Bacillus sp. OV 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BanIII, BavCI, BciBI, BcmI, Bli86I, BliRI, BspJII, BstNZ169I, SpmIBspPI Bacillus sp. d 1-34 5' GGATC 3' CCTAG 5' ---GGATCNNNN N--- 3' 3' ---CCTAGNNNNN --- 5' AclWI, AlwI, BinI, BsrWI, BstH9I, Bst31TI, Bth617I, EacIBspRI Bacillus sphaericus R 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' BspR7I Bacillus sp. R7 5' CCTNAGG 3' GGANTCC 5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5' AocI, AxyI, Bse21I, Bsu36I, Eco81I, MstII, SauI, SshAIBspST5I Bacillus sp. ST5 5' GCATC 3' CGTAG 5' ---GCATCN4N NNNN--- 3' 3' ---CGTAGN4NNNNN --- 5' BspTI Bacillus sp. RFL1265 5' CTTAAG 3' GAATTC 5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' AflIIBspT104I Bacillus sp. T104 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' AcpI, Bim19I, Bpu14I, BstBI, Csp45I, LspI, NspV, SspRFIBspT107I Bacillus sp. T107 5' GGYRCC 3' CCRYGG 5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5' AccB1I, BanI, BbvBI, Eco64I, HgiCI, HgiHI, MspB4I, PfaAIBspTNI Bacillus sp. TN 5' GGTCTC 3' CCAGAG 5' ---GGTCTCN NNNN--- 3' 3' ---CCAGAGNNNNN --- 5' BspTS514I Bacillus sp. TS514 5' GAAGAC 3' CTTCTG 5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' BspWI Bacillus sp. 3456 5' GCN7GC 3' CGN7CG 5' ---GCNNNNN NNGC--- 3' 3' ---CGNN NNNNNCG--- 5' BspXI Bacillus sphaericus X 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BliAI, BliRI, BscI, Bst28I, Bsu15I, LcaI, PgaI, Ssp27144I, ZhoIBspXII Bacillus sphaericus X 5' TGATCA 3' ACTAGT 5' ---T GATCA--- 3' 3' ---ACTAG T--- 5' AbaI, BclI, BsiQI, BstT7I, FbaI, Ksp22I, ParIBspZEI Bacillus sp. ZE 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' Bsa29I, BseCI, BspDI, BspXI, Bst28I, BstNZ169I, BsuTUI, LplI

Bsr - Bss

Enzyme PDB code Source Recognition sequence Cut IsoschizomersBsrI Bacillus stearothermophilus 5' ACTGG 3' TGACC 5' ---ACTGGN --- 3' 3' ---TGAC CN--- 5' BsrAI Bacillus stearothermophilus CPW7 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' BamNxI, BcuAI, Csp68KI, Eco47I, FssI, HgiCII, HgiJI, SinIBsrBI Bacillus stearothermophilus CPW193 5' CCGCTC 3' GGCGAG 5' ---CCG CTC--- 3' 3' ---GGC GAG--- 5' AccBSI, BstD102I, Bst31NI, MbiIBsrBRI Bacillus stearothermophilus BR 5' GATN4ATC 3' CTAN4TAG 5' ---GATNN NNATC--- 3' 3' ---CTANN NNTAG--- 5' BsrDI Bacillus stearothermophilus D70 5' GCAATG 3' CGTTAC 5' ---GCAATGNN --- 3' 3' ---CGTTAC NN--- 5' BsrFI Bacillus stearothermophilus CPW16 5' RCCGGY 3' YGGCCR 5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5' BsrGI Bacillus stearothermophilus GR75 5' TGTACA 3' ACATGT 5' ---T GTACA--- 3' 3' ---ACATG T--- 5' AauI, BsmRI, Bsp1407I, BstAUI,